TABLE 3.
Documented interactions between transport receptors and FG-Nups in S. cerevisiae
| Category | Receptor | Method(s) used to identify interactionsa |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Nup42 | Nup159 | Nup49 | Nup57 | Nsp1 | Nup100 | Nup116 | Nup145 | Nup1 | Nup2 | Nup60 | ||
| Import karyopherins | Kap95-Kap60 | A, D, E, G | D | D, E | D, E | D | D, E, G | A, C, D, E, H | D | A, D, E, G, H | A, D, E, G | E, G |
| Pse1 (Kap121) | D | E | A, E | D | E | B, D, E | B, D | A, B | B | |||
| Kap122 | C, E | D | D | |||||||||
| Kap119 (Nmd5) | D | D | D | |||||||||
| Kap104 | A, E | A | A, D | E | D, E | A, D | A | |||||
| Kap123 | A, E | E | E | A, E | D | E | C, E | A, D | E | |||
| Kap114 | G | |||||||||||
| Kap108 (Sxm1) | D, E | E | D | E | E | D | ||||||
| Mtr10 | A | D | A | A | E | A | A, D | |||||
| Import and export | Msn5 | E | E | E | E | B, E | B | B | B | |||
| Export | Xpo1 (Crm1) | A, C, E | A, C, D, E, F | A, E | A, E | E | E | E | A | A | ||
| Los1 | D, F | A, D | ||||||||||
| Cse1 | F | |||||||||||
| Unknown | Kap120 | E | E | C, E | ||||||||
| Other transport receptors | Ntf2 | D | A, D | D | A, D | A | ||||||
| Arx1 | E, G | G | G | A | G | A, E, G | A, G | G | G | G | ||
| Mex67-Mtr2 | D, E | E, G | D, E | E, G | C, D, E, F, G | G | E, G | E, G | ||||
a
A, yeast two-hybrid assay; B, fluorescence resonance energy transfer; C, copurification/biochemical pulldown; D, affinity copurification-immunoblot detection; E, affinity copurification-mass spectrometry detection; F, genetic interaction; G, reconstituted complex analysis; H, cocrystal structure analysis.