TABLE 2.
Plasmids used in this study
| Plasmid | Genotype | Source or reference |
|---|---|---|
| pRS315 | CEN LEU2 | 42 |
| pRS316 | CEN URA3 | 42 |
| pSM1107 | CEN URA3 HA-STE24 | 18 |
| pSM1282 | 2μ URA3 PPGK10HIS-HA-STE24 | 46 |
| pSM1314 | CEN URA3 RCE1-HA | 40 |
| pSM1317 | 2μ URA3 STE14 | 38 |
| pSM1585 | 2μ URA3 HsHA-STE24 | 36 |
| pSM1605 | 2μ URA3 MFA1 | 41 |
| pWS28 | 2μ URA PPGK | 52 |
| pWS335 | 2μ URA3 PPGKHIS-HA-HsRce1Δ22 | 36 |
| pWS338 | 2μ URA3 leu2-d PCUP1GST-YDJ1 | 29 |
| pWS438 | 2μ LEU2 MFA1 | 6 |
| pWS439 | 2μ LEU2 MFA1(CAMQ) | This study |
| pWS440 | 2μ LEU2 MFA1(CASQ) | 6 |
| pWS441 | 2μ LEU2 MFA1(CTLM) | 6 |
| pWS442 | 2μ LEU2 MFA1(CTVM) | This study |
| pWS479 | 2μ URA3 PPGKRCE1-HAc | 36 |
| pWS523 | CEN URA3 PGALGST-Ydj1 | 19 |
| pWS750 | CEN LEU2 PGALGFP-RAS2 | 28 |
| pWS766 | 2μ URA3 PPGKTbRce1-HA | This study |
| pWS767 | 2μ URA3 PPGKHis-HA-TbSte24 | This study |
| pWS773 | 2μ LEU2 MFA1(CTML) | This study |
| pWS774 | 2μ LEU2 MFA1(CVIM) | This study |
| pWS775 | 2μ LEU2 MFA1(CTQQ) | This study |
| pWS776 | 2μ LEU2 MFA1(CVHQ) | This study |
| pWS782 | 2μ URA3 leu2-d PCUP1GST-Tbj1 | This study |
| pWS783 | 2μ URA3 leu2-d PCUP1GST-Tbj1(C401S) | This study |
| pWS798 | 2μ URA3 PPGK TbRce1(E151A)-HA | This study |
| pWS838 | 2μ URA3 PPGK TbRce1(H184A)-HA | This study |
| pWS800 | 2μ URA3 PPGK TbRce1(H254A)-HA | This study |
| pWS801 | 2μ URA3 PPGKHis-HA-TbSte24(H286A) | This study |
| pWS805 | 2μ URA3 PPGKHis-HA-TbSte24(E287A) | This study |
| pWS802 | 2μ URA3 PPGKHis-HA-TbSte24(H290A) | This study |
| pWS880 | 2μ LEU2 MFA1(CTAQ) | This study |
| pWS888 | 2μ URA3 PPGKTbj2 | This study |
| pWS889 | 2μ URA3 PPGKTbj3 | This study |
| pWS890 | 2μ URA3 PPGKTbj4 | This study |
| pWS900 | CEN URA3 PGALGST-Tbj1 | This study |