TABLE 3.

Quantification of GFP-Ras2p plasma membrane localization

Backgrounda	CaaX protease	% PM localization ± SEMb
Wild type	Vector	93.2 ± 1.5
rce1Δ ste24Δ	Vector	37.6 ± 4.2
rce1Δ ste24Δ	S. cerevisiae Rce1	80.7 ± 1.2
rce1Δ ste24Δ	T. brucei Rce1	83.0 ± 1.4
rce1Δ ste24Δ	S. cerevisiae Ste24	42.3 ± 5.8
rce1Δ ste24Δ	T. brucei Ste24	38.6 ± 2.0

^aStrains used were EG123 (wild type) or isogenic yWS164 (rce1Δ ste24Δ) that were cotransformed with plasmids encoding GFP-Ras2p (pWS750) and, as indicated, an empty vector (pRS316) or plasmid encoding a CaaX protease (pSM1107, pSM1314, pWS766, and pWS767).

^bValues for plasma membrane (PM) localization were calculated as s/√, where s is the sample standard deviation and n is number of observations.