TABLE 4.
Coupled proteolysis-carboxyl methylation in vitro assay reveals cleavage of Tbj1 by both T. brucei CaaX proteases
| Protease | cpma | SEMb | Pc |
|---|---|---|---|
| Vector | 345.5 | 18.8 | |
| T. brucei Rce1 | 499.2 | 31.2 | 0.002 |
| T. brucei Ste24 | 454.0 | 35.7 | 0.032 |
a
Values represent mean of six data points derived from two independent experiments (three replicates per experiment).
b
The standard error of the mean was calculated from the equation s/√
, where s is sample standard deviation and n is number of observations.
c
P values calculated by analysis of variance (single factor) relative to vector condition.