Figure 1.

Characterization of human SLCO4C1 TG rats is shown. (A) Three different smaller sizes of mRNA by alternative splicing were found and mutated to avoid unusual splicing (AGGT to AGGG). (B) The mutated human SLCO4C1 cDNA was inserted into a plasmid under the proximal tubule–specific promoter. (C) Expression of human SLCO4C1 in rat organs and microdissected renal tubules examined by reverse transcriptase–PCR. Gl, glomerulus; S1, proximal tubule S1 segment; S2, proximal tubule S2 segment; S3, proximal tubule S3 segment; mTAL, medullary thick ascending limb; cTAL, cortical thick ascending limb; CCD, cortical collecting duct; IMCD, inner medulla collecting duct. (D) Immunohistochemical analysis. The human SLCO4C1 immunostains were abolished by peptide absorption. Bars = 100 μm.