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. 2009 Dec 8;10:62. doi: 10.1186/1471-2172-10-62

Figure 6.

Figure 6

IL-8 in the interaction of CD8+ T lymphocytes and endothelium. (A) The release of IL-8 from the endothelium in response to the investigated neurotransmitters (10 μM norepinephrine, 1 μM substance P and dopamine) was measured using an enzyme-linked immunoassay. The graph shows mean values and standard deviation of four independent experiments with cells from different donors. Asterisks mark statistically significant changes (p < 0.05). (B) Flow-cytometrical measurement of the CXCR1 expression on activated and naïve CD8+ T lymphocytes. The cells were distinguished for their activation state by CD25 and CD45R0 expression (left). Each subpopulation was characterized for CXCR1 expression separately (right). (C) Extravasation experiments were performed with a blockade of the IL-8 receptors on the activated CD8+ T lymphocytes by receptor-specific antibodies (abCXCR1+2). Isotypic antibodies (IgG2a) were used as control. Graphs show mean values and standard deviation of four independent experiments with cells from different donors. Asterisks mark statistically significant changes (p < 0.05).