Table 2.
Genomic Regions from Leptomonas seymouri and Crithidia sp. Sequenced in This Study.
| Species | Clone | Method | Locus | Sequence Assembly | Total Length (bp) | GenBank Accession Number | |
| Read Number | Contig Number | ||||||
| L. seymouri | 9c15 | Library screening with upstream flanking gene fragment | ama24 | 757 | 3 | 26,722 | GQ153671 |
| 1n19 | Library screening with mixed amastin fragments | ama30 | 1,118 | 1 | 38,992 | GQ153670 | |
| 1j06 | Library screening with mixed amastin fragments | ama34B | 1,243 | 1 | 39,593 | GQ153669 | |
| Crithidia sp. | 5g03 | End-sequence library: Blast match to downstream flanking gene | ama8a | 538 | 1 | 6,053 | GQ153662 |
| 6b24 | End-sequence library: Blast match to amastin | ama17 | 532 | 3 | 38,272 | GQ153665 | |
| 9h15 | End-sequence library: Blast match to downstream flanking gene | ama28 | 689 | 1 | 28,616 | GQ153667 | |
| 7m16 | End-sequence library: Blast match to amastin | ama30 | 695 | 1 | 37,238 | GQ153666 | |
| 12a17 | End-sequence library: Blast match to downstream flanking gene | ama31a | 569 | 3 | 43,87* | GQ153668 | |
| 5p16 | End-sequence library: Blast match to downstream flanking gene | ama34Aa | 628 | 2 | 39,331 | GQ153663 | |
| 2 e16 | End-sequence library: Blast match to amastin | ama34B | 624 | 1 | 37,400 | GQ153661 | |
| 1m01 | End-sequence library: Blast match to upstream flanking gene | ama34Ea | 389 | 2 | 37,943 | GQ153660 | |
| 6b12 | End-sequence library: Blast match to upstream flanking gene | ama36a | 696 | 1 | 31,933 | GQ153664 | |
NOTE.—The total sequence length of Crithidia sp. clone 12a17 exceeds the theoretical maximum of 40 kbp because it contained repetitive material that was assembled multiple times; this did not affect evaluation of the amastin locus because this was nonrepetitive. Blast, basic alignment search tool.
a
These loci lacked amastin in L. seymouri or Crithidia sp.