Skip to main content
. 2009 Sep 11;113(1):27–36. doi: 10.1093/toxsci/kfp212

FIG. 3.

FIG. 3.

Ligand activation of PPARβ/δ and inhibition of COX-2 inhibits cell proliferation in keratinocyte cancer lines. The 308, SP1, and Pam212 keratinocyte cancer lines were cultured in low (0.05mM) calcium keratinocyte culture medium containing GW0742 (1μM), nimesulide (100μM), or the combination of both (n = 3 replicates per treatment group). Average cell number for (A) 308 keratinocytes, (B) SP1 keratinocytes, and (C) Pam212 keratinocytes were quantified using a Coulter counter. Values represent mean ± SEM. *Significantly different as determined by ANOVA and post hoc testing, p ≤ 0.05. Cell cycle progression was determined by flow cytometry after 72 h with the indicated treatment in (D) 308 keratinocytes, (E) SP1 keratinocytes, and (F) Pam212 keratinocytes. Values are the percentage of cells within a specific phase of the cell cycle and represent the mean ± SEM from three independent replicates. *Significantly different, p ≤ 0.05. *,+Significantly different than control and individual treatments, p ≤ 0.05.