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. 2009 Oct 4;113(1):198–206. doi: 10.1093/toxsci/kfp226

FIG. 2.

FIG. 2.

Recombinant FGF10 protein significantly increases prostatic budding in UGS organ culture, and TCDD blocks this effect. UGS from E14.5 male C57BL/6J mouse fetuses were incubated for 3 days in organ culture medium containing vehicle, recombinant FGF10 protein (300 ng/ml), TCDD (1nM), or FGF10 + TCDD. Media and supplements were replenished after the second day of culture. At the end of the incubation, UGE was separated from UGM and visualized by scanning electron microscopy. (A) Micrographs of one UGE from each exposure group are shown. Prostatic buds are pseudocolored red. (B) Prostatic buds were counted using micrographs of each UGE that were taken from four separate angles in order to ensure that all prostatic buds present were counted. Results for the total number of prostatic buds per UGS are mean ± SE of n ≥ 6 independent samples per group from at least three separate litters. The presence of an asterisk indicates a mean that is significantly different from the vehicle-treated group (control, p < 0.05). The presence of a dagger indicates it is significantly different from 1nM TCDD (control, p < 0.05).