Fig. 1.

Coefficients of variation (CV)s for metabolites measured in fresh serum. The CVs were calculated for each metabolite measured (e.g. each fatty acid within each lipid class) in 5 replicates of the same fresh serum sample from Experiment 1. The CVs were plotted in decreasing order for all metabolites. Low abundance metabolites (e.g. DG20:4n3, PE14:1n5) had higher CVs whereas high abundance metabolites (e.g. TG18:1n9, CE16:0) had very low CVs as low as 0.01 and 0.02. This illustrates the high reproducibility of the analytical method for metabolites of biological significance in humans