Table 1. Results of the ultradeep sequencing screening.

Sample	Total Reads	Total Bases	Average Read Length (bp)	Aligned Reads	Positions with High Substitution Frequency (>0.1%)			Positions with Low Substitution Frequency (<0.1%)
					SNPs	Clonal Mutations	Errors
CC	460,584	89,958,949	195.3	99.8%	2	2	20	1,221
NC	429,940	83,376,393	193.9	98.9%	2	0	18	1,215
PBL	496,358	96,210,962	193.8	99.8%	2	0	35	1,151
H-PBL	459,691	88,625,322	192.8	99.4%	2	0	38	1,157

For each sample, the total number of sequence reads and sequenced bases are shown, together with the average length of the reads and the percentage of reads aligned to the reference sequence. The latter correspond to the fraction of reads that passed the quality filter of 454 sequencing. Reported also are the positions of eUCR41 with substitutions at high (>0.1%) and low (<0.1%) frequency. The threshold of 0.1% represents the detection power of 454 sequencing.