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. 2009 Dec 22;4(12):e8290. doi: 10.1371/journal.pone.0008290

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Rajendran et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Filter-grown MDCK cells were immunostained for apical marker protein, GP135 (green) and flotillin-1 (blue) or with Phalloidin to stain actin (red) and flotillin-2 (blue). Note that GP135 marks exclusively the apical membrane while flotillin-1 and -2 are mainly associated with intracellular vesicles. Actin, on the other hand marks both apical and basolateral membranes.