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. 2009 Dec 24;4(12):e8447. doi: 10.1371/journal.pone.0008447

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Arazi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The within-day variation was computed as the difference between each FACS measurement and its corresponding daily average (i.e. the average of the 2 measurements taken for the relevant day, cell subset and treatment group), divided by this average. The between-days variation was computed as the difference between each daily average and the overall average of the series (i.e., the average of all the measurements related to a cell subset and treatment group), divided by the overall average. The division by averages serves as a normalization step, allowing to compare all cell subsets together. Horizontal lines represent the medians. The statistical significance of the differences between the within-day and between-days variations was estimated using the non-parametric Mann-Whitney U-test, resulting with P<0.001 for each of the 3 treatment groups. For the non-injected mice, the difference between the within-day and between-days variations was not statistically significant.