Figure 2.

Pluripotency and differentiation. (a) Western blot analysis for Oct 4 shows strong expression in ESD0 and F9 cells and its absence from ESD14 cells. Expression of β-actin confirms equal loading. (b) Semiquantitative RT-PCR analysis shows expression of transcripts of TBra, AFP, and NF-68, markers of mesoderm, endoderm, and ectoderm, respectively, in differentiated ESD14 cells. In contrast, these transcripts are not detected in undifferentiated ESD0 and F9 cells. β-actin was used as loading control.