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. 2009 Dec;11(12):1318–1328. doi: 10.1593/neo.91084

Figure 4.

Figure 4

ALDH activity in Twist-dysregulated breast cells. Dot plots of various cell lines analyzed by flow cytometry for ALDH activity. Cells were treated with ALDEFLUOR in the presence or absence of ALDH inhibitor DEAB. After treatment, the samples were analyzed by flow cytometry for the presence of ALDH bright cells. The ALDH bright region was based on the control DEAB sample that was gated to have less than five events. The cell percentage numbers for ALDH positivity are indicated in the bottom right of each histogram. The values presented are the averages of three independent experiments. The analyzed data are represented as dot plots of (first row) parental MCF-10A and MCF-7 breast cancer cell lines, (second row) stable Twist-overexpressing MCF-10A/Twist and MCF-7/Twist breast cancer cell lines, (third row) breast cell lines MCF-10A and MCF-7 transiently transduced with Twist-expressing retroviral constructs, and (fourth row) Twist knockdown breast cancer cell lines MCF-10A/Twist and MCF-7/Twist. Results are representative of five separate experiments.