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. 2009 Dec;11(12):1329–1339. doi: 10.1593/neo.91110

Figure 2.

Figure 2

A representative result of bisulfite sequencing. Each panel represents a schematic representation of the genome structure of each gene including their 5′ CpG islands. Exons and untranslated regions are represented by filled or open boxes, respectively. The transcription initiation site is represented by +1. Expanded view shows the position of CpG islands and the region analyzed by bisulfite sequencing. Vertical marks represent individual CpG dinucleotides and their spacing accurately reflects the CpG density of the region. MSP primers are represented by horizontal arrows in the panels. Primer sets M and U were designed for the same CpG dinucleotide (indicated by an asterisk). Methylation profiles of the treated (5Aza-dC) and untreated (mock) cell lines are indicated in the lower part of the panels. Each row represents one sequenced clone, and open and filled circles represent unmethylated and methylated CpG dinucleotides, respectively. (A) Bisulfite sequencing of CRABP2 in the FaDu cell line. (B) Bisulfite sequencing of MX1 in the FaDu and UM-SCC-14A cell lines. (C) Bisulfite sequencing of SLC15A3 in the FaDu and UM-SCC-38A cell lines.