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. 2009 Dec 30;4(12):e8494. doi: 10.1371/journal.pone.0008494

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The pre-immune OD 450 nm values found by ELISA for anti-nuclear antibodies (ANA), anti-Sm and anti-RNP are shown separately in light blue because of the high levels of antibodies in some rabbits prior to immunization. All post-immunization responses are given as ΔOD (the OD 450 in post-boost sera minus the OD 450 in pre-immune sera).