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. 2009 Dec 24;4(12):e8462. doi: 10.1371/journal.pone.0008462

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) One cell embryos were injected with morpholinos, and subsequently harvested at Stage 39. Equal amounts of protein from Control-MO (left) or SENP3-MO (right) embryos were subjected to SDS-PAGE and Western blotting with anti-SENP3 or -actin antibodies. (B) Samples as in (A) were subjected to SDS-PAGE and Western blotting with anti-SUMO-2 and –SUMO-1 antibodies, as indicated. Note the substantial accumulation of high molecular weight SUMO-2-conjugated species after SENP3 knockdown, while there is essentially no change in SUMO-1 conjugates. (C) Morphological defects of SENP3-MO injected embryos were categorized according to defects in body patterning and eye development, as indicated (lower three panels). The morphology of embryos injected with Control-MO is shown in the upper panel. (D) Quantitation of morphological body deformities of SENP3-MO injected embryos. Bars indicate standard error of the mean values. p values are indicated.