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. 2009 Dec 24;4(12):e8445. doi: 10.1371/journal.pone.0008445

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A–C) miR-24 over-expression down-regulated DHFR protein levels in three cancer cell lines independent of p53 function; in colon cancer HCT 116 (wt-p53) (A), osteosarcoma cell line U2-OS (wt-p53) (B), and HCT-116 (null-p53) cancer cell lines (C). Oligofectamine alone (oligo) and non-specific miRNA (neg) were used as the negative controls. siRNA specific against DHFR (siDHFR) was the positive control. (D–E) The levels of DHFR mRNA in HCT-116 and U-2 OS cells were determined by real time qRT-PCR analysis, GAPDH was used as an internal standard for normalization (data are shown as mean±SD. *, P<0.05). (F) miR-24 not only inhibited cell growth but also induced MTX resistance in a HCT-116 colorectal cancer cell line.