Fig. 4.

In vitro characterization of mutant FimH strains. (A) Type 1 pilus phase assay. Mutations are shown at the bottom. The phase orientation in a population of cells grown under type 1 inducing conditions is shown. Bands corresponding to the ON and OFF orientation are indicated. (B) Immunoblot for fimbrial proteins. Bands corresponding to FimA, FimC, and FimH are indicated. (C) In vitro binding assays and in vitro biofilm formation assay. Mutations present are shown on the x axis. “ON” indicates the phase locked ON strain. Binding titers (log base 2) to immobilized BSA-mannose (black bars) and hemagglutination (HA) titers (gray bars) are shown on the left y axis. Relative biofilm formation (white bars) at 24 h for the mutants is plotted on the right y axis, and has been arbitrarily normalized to 100% for the wt-control or wt-ON strains. Data from a single experiment are shown.