Figure 3.

Misexpression of dLMO using Ser-G4 to drive expression of EP 1394 at the D-V boundary during third instar. (A) Immunohistochemistry using an antibody against the Ct protein demonstrates loss of continuity in the developing wing margin of third instar wing discs. Anti-Ct antibody, similar to wg-lacZ (Fig. 4A), stains the D-V boundary in wild-type wing discs. The arrows point out the remaining Ct-positive cells forming islands along the D-V boundary. n, notum precursor region; h, hinge precursor region; d, dorsal wing precursor compartment; v, ventral wing precursor compartment. (B) The adult wing produced when dLMO is misexpressed at the D-V boundary. The wing margin shows a substantial loss of tissue. (C) The adult wing from a Bx³ mutant displays a similar phenotype of loss of wing margin tissue.