FIGURE 1.

Xylosyltransferase activities of C. neoformans. Total membranes prepared from CAP67 cells were assayed as described under “Experimental Procedures” with UDP-[¹⁴C]Xyl in the presence (+) or absence (−) of the α-1,3-Man₂ acceptor and MnCl₂ cofactor as indicated. An autoradiograph of the products resolved by TLC is shown, with the migration positions of free Xyl and α-1,3-Man₂ standards indicated at the right. O, origin; F, solvent front. In this and subsequent figures, the filled arrowhead indicates the product of the manganese-dependent xylosyltransferase activity, and the open arrowhead indicates the products of unrelated xylosyltransferase activities (see “Results”).