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Xylosyltransferase activities in CXT1 and CXT2 mutants. Xylosyltransferase activity assays of membranes from the strains indicated were performed with UDP-[14C]Xyl and α-1,3-Man2 in the presence (+) or absence (−) of MnCl2. Only the relevant region of the TLC plate is shown; no signal was detected in other regions beyond minor amounts of free Xyl (see Fig. 1). Symbols are as in Fig. 1.
