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. 2009 Oct 28;284(52):36118–36127. doi: 10.1074/jbc.M109.056226

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Competition of Xpt1p activity. Xylosyltransferase activity assays were performed using detergent extracts of KN99α cxt1Δ cxt2Δ cells (see “Experimental Procedures”). All reactions contained standard amounts of UDP-[¹⁴C]Xyl, α-1,3-Man₂, and MnCl₂ with varying amounts of UDP-Xyl (A) or GDP-Man (B). Lane 1, 0 μm; lane 2, 1 μm; lane 3, 5 μm; lane 4, 10 μm; lane 5, 25 μm; lane 6, 50 μm; lane 7, 75 μm; lane 8, 100 μm; lane 9, 250 μm; lane 10, 500 μm. Only the region of the TLC plate with the Xpt1p product is shown; no signal was detected in other regions. F, solvent front.