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Donor substrates of Xpt1p. Xpt1p activity assays were performed as in Fig. 2, using total membranes from wild-type, mutant, or complemented mutant strains as indicated. All reactions contained α-1,3-Man2, MnCl2, and the radiolabeled nucleotide sugar donor indicated. Labels are as in Fig. 1, although the arrowheads refer only to lanes 1–3; the identity of the radiolabeled products in lanes 4–9 has not been determined.
