FIGURE 2.
Suppression of Txnip is independent of cGMP synthesis or ambient O2 concentration. A, RAW264.7 cells were stimulated with LPS/IFN-γ for 16 h in either ambient O2 (21%) or physiological O2 (2%), and the expression of Txnip and iNOS was assessed by Western blotting. B, RAW264.7 cells were stimulated with LPS/IFN-γ for 16 h in the absence or presence of the soluble guanylyl cyclase inhibitor ODQ, and the expression of Txnip/iNOS was assessed by Western blotting. C, RAW264.7 cells were stimulated with LPS/IFN-γ for 16 h or treated with 8-bromo-cGMP.