FIGURE 5.

Txnip facilitates nitrosative stress in iNOS-transfected HEK293 cells. A, HEK293 cells were incubated with 2 mm DETA-NO and/or 2 μm auranofin for 16 h, and lactate dehydrogenase (LDH) release was measured. B–E, HEK293 cells were transfected with either empty pCMV or pCMV-Txnip plasmid (0, 2, or 4 μg) and empty pLP or pLP-iNOS(WT) plasmid (3 μg). After 28 h, media nitrite (NO₂⁻) (B) and lactate dehydrogenase release (C) were measured. D, cells were transfected for 20 h as in B, and cellular uptake of [³H]thymidine was assayed. E, samples from above were visualized by light microscopy prior to lactate dehydrogenase measurement. Shown are cells transfected with pLP-iNOS(WT) and either empty pCMV (left panel) or pCMV-Txnip (right panel) plasmid.