FIGURE 2.

Phenotypical analysis of the tic-2 mutant in response to iron status. A, representative 4-week-old Col and tic-2 plants grown on soil in a greenhouse and irrigated with water (control) or irrigated with iron-EDDHA 600 μm (+Fe). B, determination of pigment content in 4-week-old Col and tic-2 plants grown on soil in a greenhouse and irrigated with water. FW, fresh weight. C, determination of iron content in the leaves of 4-week-old Col and tic-2 plants grown on soil in a greenhouse and irrigated with water. Shown are the means ± S.D. of nine measurements (three replicates/experiment performed three times). D, iron sensitivity of plants grown as followed. The plants were grown in vitro during 2 weeks on medium without the addition of iron (−Fe), with the addition of iron-EDTA 50 μm (control), or with the addition of Fe- EDTA 500 μm (+Fe). Col is on the left half of each panel, and tic-2 on the right half. E, mean fresh weight of one plantlet. The plants were grown as described for D. The weight of a plantlet was calculated as followed: weight of a plantlet = (fresh weight of plantlets of a genotype from the same plate)/(number of plantlets of a genotype from the same plate). B and E, shown are the means ± S.D. (n = 3). B, C, and E, *, p < 0.05; **, p < 0.01; black and white bars represent, respectively, Col and tic-2.