FIGURE 5.
Involvement of TIC and the IDRS cis-element in the AtFer1 regulation. The tic-2 mutant was crossed with the transgenic lines pIDRS::GUS Col and pIDRS*::GUS Col to produce the lines pIDRS::GUS tic-2 and pIDRS*::GUS tic-2. The plants were grown in vitro under iron sufficiency during 10 days before being transferred for 4 days on iron-starved medium (−Fe). For the iron excess treatment, part of the iron-starved plants was sprayed with iron citrate 500 μm (+Fe). Leaves from plants grown on the different iron conditions were collected 5 h after the iron spray. The GUS RTL was measured in leaves by Q-PCR, using PP2 (At1g13320) as a housekeeping gene (58). A, RTL of the GUS reporter gene in iron deficiency. B, RTL of the GUS reporter gene in response to iron excess. Shown are the means ± S.D. with n = 3 (A and B).