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. 2009 Sep 24;284(52):36312–36323. doi: 10.1074/jbc.M109.049817

FIGURE 2.

FIGURE 2.

Creation and characterization of MHC-DGAT1 transgenic mice. A, DGAT1 transgene contains (from the 5′- to 3′-end) the 5.5-kb MHC promoter, a human DGAT1 cDNA containing its own initiation and termination codons, and the genomic sequence of human growth hormone (hGH) containing the last 3 exons and 2 introns as indicated. B, tissue distribution of the DGAT1 transgene mRNA levels measured by reverse transcription and PCR amplification. C, total DGAT activity levels in membrane fractions of heart muscles from WT and transgenic mice (n = 3 in each group). D–G, TG, FFA, DAG, and ceramide contents in heart muscles isolated from WT and transgenic mice (n = 5–6). *, p < 0.05; **, p < 0.01.