TABLE 2.
The EC50 of glycine and IC30 values (±S.E.) for NAGly and NALAla inhibition of glycine transport by wild type and GLYT2 EL4 mutant transporters
| Transporter | EC50 of glycine | IC30 |
|
|---|---|---|---|
| NAGly | NALAla | ||
| μm | μm | ||
| GLYT2 | 18 ± 2 | 3.4 ± 0.6 | 5.9 ± 0.7 |
| GLYT1 | 22 ± 2 | >30 | >30 |
| E530H | 11.2 ± 0.9 | 2.1 ± 0.7 | |
| R531L | 10 ± 4 | 13 ± 2 | 14 ± 1 |
| K532G | 39 ± 2 | 9 ± 1 | 12 ± 1 |
| N534D | 19 ± 2 | 5 ± 1 | |
| I535V | 13 ± 2 | 2.2 ± 0.4 | |
| E536S | 13 ± 3 | 2.3 ± 0.5 | |
| N537R | 10 ± 1 | 4.9 ± 1.5 | |
| Q541H | 12.7 ± 0.8 | 2.6 ± 0.4 | |
| I545L | 8.4 ± 0.8 | 42 ± 7 | 21 ± 1 |
| V549A | 9 ± 1 | 1.3 ± 0.2 | |
| R556L | 6 ± 1 | 8 ± 1 | |