FIGURE 6.

KLF11 interacts with the p300 coactivator in pancreatic L3.6 cells. A, FLAG-tagged KLF11 and His-tagged p300 expression vectors were transfected into L3.6 cells. Immunoprecipitation (IP) analysis was performed with α-FLAG antibody, and the precipitates were immunoblotted (IB) with α-His antibody. B, L3.6 total lysates were immunoprecipitated with α-KLF11 and α-p300 and then probed with α-KLF11 or α-p300. C, FLAG-p300 was cotransfected with His-tagged KLF11 expression constructs spanning the N-terminal repressor domains R1, R2, R3 (N-Term), or C-terminal zinc finger domains (32). Only the C-terminal domain interacted with p300, although comparable levels of both KLF11 constructs were produced.