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Tb-Nbp35, TbCfd1, and Tb-Nfs1 but not Tb-Mtu1 are essential for normal growth of procyclic T. brucei. Growth curve in the presence and absence of tetracycline (+, -Tet) of representative clonal Tb-Nbp35, Tb-Cfd1, Tb-Nfs1, and Tb-Mtu1 RNAi cell lines. Insets: Northern blots of the corresponding mRNAs. The RNA from induced cells was isolated at 72 h, the time of the growth arrest (arrows). The rRNAs in the lower panel of the insets serve as loading controls.
