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. 2009 Oct 29;284(52):36491–36499. doi: 10.1074/jbc.M109.064527

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Ablation of Tb-Nfs1 and Tb-Mtu1 but not of Nbp35 and Tb-Cfd1 causes accumulation of non-thio-modified mitochondrial tRNA^Trp. RNA isolated from total cell (tot) and digitonin-extracted mitochondrial pellets (mt) of the indicated uninduced (-Tet) and induced (+Tet, 72 h for Tb-Nfs1, Tb-Nbp35, Tb-Cfd1, and 120 h for Tb-Mtu1) RNAi cell lines were separated on APM-containing polyacrylamide gels. Positions of thio-modified (thio-tRNA) and non-thio-modified tRNAs (non-thio-tRNA) are indicated. The top panel shows the ethidium bromide-stained gel. The bottom panel shows the corresponding Northern blot probed for the tRNA^Trp. The numbers at the bottom indicate the percentage (±S.E.) of non-thio-modified mitochondrial tRNA^Trp before (-Tet) and after (+Tet) induction of RNAi.