FIGURE 4.

Chemical shift resonance assignments and structural analysis of the 14-mer peptide (STKAAKKPEAKREA), by two-dimensional TOCSY and NOESY spectroscopy. A, ¹H chemical shift assignments were successfully determined using the inter- and intra-residual correlations in TOCSY and NOESY spectra. All ¹H chemical shifts are tabulated in Table 3. B, inter-residual NOE signals were found between ¹H^δ of Pro²⁸ and ¹H^γ of Lys²⁶, ¹H^δ of Pro²⁸ and ¹H^β of Lys²⁷, ¹H^γ of Pro²⁸ and ¹H^α of Lys²⁶, and ¹H^δ of Pro²⁸ and ¹H^α of Lys²⁷.