TABLE 1.
Binding constants (KA), association rate constant (kon), and dissociation rate constant (koff) of specific and nonspecific GAG binding affinity of vaccinia viral envelope proteins sA27-aa and D8
Binding constants (KA) were calculated from the ratio kon/koff. Data represent the means ± S.D. of at least four independent experiments at five different protein concentrations.
| Specific | kon (×103m−1s−1) | koff (×10−6s−1) | KA (×108m−1) |
|---|---|---|---|
| sA27-aa, HP | 1.71 ± 0.42 | 13.7 ± 2.3 | 1.25 ± 0.80 |
| D8, CS | 11.9 ± 1.5 | 36.8 ± 26.6 | 3.23 ± 0.20 |
| Nonspecific | kon (×103m−1s−1) | koff(×10−3s−1) | KA (×105m−1) |
|---|---|---|---|
| sA27-aa, CS | 2.58 ± 0.04 | 15.6 ± 0.7 | 1.65 ± 0.40 |
| D8, HP | 1.45 ± 0.04 | 1.60 ± 0.35 | 9.06 ± 0.17 |
| T22K/A25K, CS | 3.57 ± 0.02 | 5.64 ± 0.18 | 6.33 ± 0.30 |