TABLE 2.
Heparin binding constants (KA), association rate constant (kon), and dissociation rate constant (koff) of sA27-aa mutants determined by SPR
Binding constants (KA) were calculated from the ratio kon/koff. Data represent the means ± S.D. of at least four independent experiments at five different protein concentrations.
| kon (×103m−1s−1) | koff (×10−3s−1) | KA (×106m−1) | |
|---|---|---|---|
| A series | |||
| K23A | 1.60 ± 0.22 | 2.20 ± 0.14 | 0.72 ± 0.07 |
| K26A | 2.00 ± 0.27 | 1.70 ± 0.71 | 1.18 ± 0.53 |
| K27A | 1.10 ± 0.19 | 1.90 ± 0.13 | 0.58 ± 0.05 |
| K31A | 1.20 ± 0.04 | 1.20 ± 0.15 | 1.00 ± 0.12 |
| R32A | 0.97 ± 0.03 | 1.40 ± 0.13 | 0.69 ± 0.07 |
| Scrambled | |||
| K3A3 | 2.56 ± 0.36 | 0.46 ± 0.03 | 5.57 ± 0.78 |
| K series | |||
| E29K | 26.5 ± 16.0 | 1.92 ± 0.11 | 13.80 ± 1.80 |
| A30K | 12.8 ± 0.63 | 3.76 ± 0.10 | 3.40 ± 0.23 |
| kon (×103m−1s−1) | koff (×10−6s−1) | KA (×109m−1) | |
|---|---|---|---|
| T22K | 3.88 ± 0.15 | 2.21 ± 0.10 | 1.75 ± 0.50 |
| A25K | 2.55 ± 0.10 | 3.40 ± 0.12 | 0.75 ± 0.20 |
| kon (×103m−1s−1) | koff (×10−7s−1) | KA (×1011m−1) | |
|---|---|---|---|
| T22K/A25K | 26.7 ± 2.3 | 1.39 ± 0.01 | 1.90 ± 0.40 |