Binding analyses of Brd4 BD1 and BD2 bromodomains to acetylated lysine peptides by isothermal titration calorimetry. A, peptide sequences used within this study. B, control measurement of a Brd4 BD1 titration in buffer solution. C, ITC measurements of BD1 to H3-K(ac)14 revealed a dissociation constant of 118 μm. D, interaction of BD1 with H4-K(ac)5. E, binding of BD2 to acetylated cyclin T1 peptides K(ac)390. F, binding of BD2 to CycT1-K(ac)404. The thermodynamic parameter and dissociation constants are listed in Table 2.