FIGURE 5.

c-Src localization to the mitochondria is promoted by overexpression of EGFR and kinase activities of EGFR and c-Src. 10T1/2 cells with endogenous levels of EGFR and overexpressed wt c-Src (A) or 10T1/2 cells co-overexpressing EGFR and kinase-defective c-Src (B) were treated as in Fig. 1 and immunostained for c-Src. C and D, 10T1/2 cells co-overexpressing EGFR and wt c-Src were treated with 10 μm PP2 (C) or 5 nm AG1478 (D) for 1 h before the addition of EGF. The percentage of cells with merge of the red and green stains was quantified. Results are expressed as the mean ± S.D. of 2 experiments (50 cells were counted per experiment).