TABLE 1.
In vitro H2 production activity with wild type and SDM variants of HydA1 and PetF
Each value represents the mean of at least three independent assays. Underlined values indicate a significantly negative influence on HydA1 activity. Vmax and H2 production (H2-prod.) is given as μmol of H2 mg−1 min−1. The Km values refer to micromoles of ferredoxin. WT, wild type.
| HydA1 variant | Vmax (MV) | H2 prod. (40 μm PetF) | PetF variant | Vmax | Km |
|---|---|---|---|---|---|
| WT | 730 ± 54 | 278 ± 19 | WT | 435 ± 21 | 21 ± 2 |
| R96Q | 277 ± 22 | 147 ± 15 | D56N | 243 ± 10 | 39 ± 2.0 |
| R349Q | 476 ± 31 | 245 ± 7 | E59Q | 345 ± 26 | 27 ± 3.1 |
| R353Q | 667 ± 25 | 244 ± 12 | E60Q | 571 ± 38 | 36 ± 6.2 |
| R379Q | 370 ± 17 | 205 ± 10 | D64N | 352 ± 31 | 45 ± 7.1 |
| K179Q | 588 ± 26 | 182 ± 5 | D90N | 463 ± 22 | 50 ± 2.2 |
| K262Q | 667 ± 38 | 214 ± 10 | F93L | 138 ± 39 | 86 ± 12 |
| K433Q | 712 ± 12 | 165 ± 15 | S92A | 435 ± 41 | 30 ± 2.9 |
| K396Q | 714 ± 22 | 51 ± 17 | D95N | 406 ± 35 | 53 ± 11 |
| K397E | 770 ± 34 | 17 ± 15 | E122Q | 115 ± 29 | 519 ± 87 |
| K396Q/K397Q | 715 ± 25 | 23 ± 9 | E123Q | 500 ± 28 | 41 ± 3.3 |