Skip to main content
. Author manuscript; available in PMC: 2011 Jan 5.
Published in final edited form as: Mutat Res. 2010 Jan 5;683(1-2):50–56. doi: 10.1016/j.mrfmmm.2009.10.007

Table 4.

Features of Me-lex induced p53 mutations in rad30 (polη) yeast strain

N° mutation sequenze context
5′->3′a 		base pair
changes		 codon Aminoacid
substitution
#1 GGA AAT TTG AT>GC 200 Asn>Asp
#2 TAC AAG CAG AT>TA 164 Lys>Stop
#3 GGA AAT TTG AT>GC 200 Asn>Asp
#4 GGT TTC CGT AT>GC 109 Phe>Ser
#5 CAG CAT CTT GC>TA 193 His>Asn
#6 AAT TTG CGT AT>TA 201 Leu>Stop
#7 AAT TTG CGT AT>TA 201 Leu>Stop
#8 GAG AAT CTC AT>TA 288 Asn>Tyr
#9 b AAT TTG CGT AT>TA 201 Leu>Stop
#10 CAG GGC AGC GC>TA 105 Gly>Cys
#11 GAG CGC TTC GC>AT 337 Arg>Cys
#12 AAT TTG CGT AT>TA 201 Leu>Stop
#13 GAG AAT CTC AT>TA 288 Asn>Tyr
#14 CAG CAC ATG AT>GC 168 His>Arg
#15 GGA AAT TTG AT>GC 200 Asn>Asp
#16 CAG CTG TGG AT>TA 145 Leu>Gln
#17 TGT AAC AGT AT>GC 239 Asn>Asp
#18 TAT TTC ACC AT>TA 328 Phe>Tyr
#19 TTT TGC CAA AT>GC 135 Cys>Arg
#20 b CAC CAT GAG AT>TA 179 His>Gln
#21 CTT ATC CGA AT>GC 195 Ile>Thr
#22 Cga Gtg>Cg(+1g)A Gtg +1G insertion
#23 AAG ATG TTT AT>CG 133 Met>Arg
#24 GTG GTG CCC AT>CG 218 Val>Gly
#25 AAT TTG CGT AT>CG 201 Leu>Trp
#26 AAC TAC ATG AT>CG 236 Tyr>Ser
#27 GCC AAG ACC AT>CG 138 Lys>KQ
#28 ATG TTT TGC AT>CG 134 Phe>Leu
#29 b CTT ATC CGA AT>GC 195 Ile>Thr
#30 GGC ATG AAC AT>GC 246 Met> Thr
#31 CGC TGC CCC AT>GC 176 Arg>Cys
#32 ATC ATC ACA AT>GC 255 Ile>Thr
#33 AAG AAG AAA AT>GC 320 Lys>Glu
#34 GAG TAT TTG AT>GC 205 Cys>Tyr
#35 ATG TTC CGA AT>GC 341 Phe>Ser
#36 GAA GGA AAT AT>GC 199 Cys>Cys silent
#37 TTT TGC CAA AT>GC 135 Cys>Arg
#38 TAT TTC ACC AT>GC 328 Phe>Leu
#39 AAT TTG CGT AT>TA 201 Leu>STOP
#40 CTC AAG GAT AT>TA 351 Lys>STOP
#41 CTC AAC AAG AT>TA 131 Asn>Tyr
#42 AAT TTG CGT AT>TA 201 Leu>STOP
#43 CGT CTG GGC AT>TA 111 Leu>Gln
#44 AAT TTG CGT AT>TA 201 Leu>STOP
#45 GGA AAT TTG AT>TA 200 Asn>Tyr
#46 AAT TTG CGT AT>TA 201 Leu>STOP
#47 CTC AAC AAG AT>TA 131 Asn>Tyr
#48 AAT TTG CGT AT>TA 201 Leu>STOP
#49 b TAT TTG GAT AT>TA 206 Leu>STOP
#50 b CGT CTG GGC AT>TA 111 Leu>Gln
#51 GAC CGG CGC GC>AT 282 Arg> Gln
#52 GGC TCT GAC GC>AT 227 Ser>Phe
#53 TTT CGA CAT GC>AT 213 Arg>STOP
#54 CCT TCC CAG GC>AT 99 Ser>Ser silent
#55 AAC AGT TCC GC>AT 240 Ser>Asn
#56 AAG CGA GCA GC>AT 306 Arg>Gln
#57 CGC GTC CGC GC>AT 157 Val>Ile
#58 GAG GTT GTG AT>TA 172 Val>Asp
#59 CGC ACA GAG GC>AT 284 Thr>Ile
#60 CCT TCC CAG GC>AT 99 Ser>Phe
#61 b ATG ACG GAG GC>AT 170 Thr>Met
#62 TTT CGA CAT GC>CG 213 Arg>Pro
#63 TTT TGC CAA GC>TA 135 Cys>STOP
#64 CAG GGC AGC GC>TA 105 Gly>Val
a

in bold: nucleotide position; underlined: codon triplet where the mutation causes the indicated aminoacid change.

b

mutations found in mutant isolated at 12mM Me-lex, all the other 57 mutations were found in mutants isolated at 6mM Me-lex.