Table 5.
Class of base pair substitutions of Me-lex induced p53 mutations in different genetic backgrounds
| WTa | rad30 | WT vs rad30b | |
|---|---|---|---|
| GC-targeted | 19(38%) | 16 (25%) | NS |
| GC>AT | 7 (14%) | 11 (17%) | NS |
| GC>TA | 6 (12%) | 4 (6%) | NS |
| GC>CG | 6 (12%) | 1 (2%) | p=0.043 |
| AT-targeted | 31 (62%) | 47 (75%) | NS |
| AT>TA | 21 (42%) | 23 (37%) | NS |
| AT>GC | 6 (12%) | 18 (29%) | p=0.039 |
| AT>CG | 4 (8%) | 6 (9%) | NS |
| 50 (100%) | 63 (100%) |
a
data from (8)
b
Fisher’s exact test: events in a specific class vs total
Of the 63 base pair substitutions reported, 57 were found in mutants isolated at 6 mM Me-lex, while the remaining 6 mutations were found in mutants isolated at 12mM.
Note that the statistically significant differences remain even if the six mutations found at 12mM are excluded (p=0.048; p=0.034 respectively).