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. Author manuscript; available in PMC: 2010 Jun 10.
Published in final edited form as: Oncogene. 2009 Dec 10;28(49):4317–4325. doi: 10.1038/onc.2009.287

Figure 5. K269R cyclin D1 transforms NIH-3T3 cells.

Figure 5

A. Anchorage –independent growth of NIH-3T3 cells stably expressing WT cyclin D1, D1-T286A, D1-K269R or pBabe-puro vector was analyzed by growth in soft agar. Colonies were visualized by microscopy. B. Quantification of triplicate samples shown in A. C. Foci formation ability of NIH-3T3 cells stably expressing WT cyclin D1, D1-T286A and D1-K269R was determined by Giemsa stain of foci grown for 21 days.