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. Author manuscript; available in PMC: 2010 Dec 22.
Published in final edited form as: Biochemistry. 2009 Dec 22;48(50):11872–11882. doi: 10.1021/bi9014488

Figure 5. Inhibition of A/M2 channel activity by consecutive application of BL-1743 and amantadine.

Figure 5

A–D; Representative traces of the A/M2 channel activity modulated by consecutive application of BL- 1743 and amantadine. A/M2 channel activity was induced by the application of the activating solution (pH 5.5, red horizontal bar above the trace). The currents were first inhibited by the application of the activating solution (pH 5.5) containing various concentrations of BL-1743 (3–1000 μM, green horizontal bar) for 60 sec. After maximal inhibition of the channel activity was achieved, amantadine (100 μM) was substituted for BL-1743 (3–1000 μM) in the activating solution for 90 sec (yellow horizontal bar). The inhibitors were washed out for 5 min by the non-activating solution (pH 8.5, second blue horizontal bar) and the pH-induced activity of A/M2 channels was measured again (second red horizontal bar). Note the biphasic current upon application of amantadine during washout of a saturating concentration of BL-1743 (during yellow bar in D).