Skip to main content
. Author manuscript; available in PMC: 2010 Dec 14.
Published in final edited form as: Anal Chem. 2009 Dec 15;81(24):10019–10028. doi: 10.1021/ac901897x

Figure 2.

Figure 2

Gas-phase HDX of peptides with ND3 gas in the transfer TWIG. (a) The deuterium labeling of Leucine enkephalin, Glu-fibrinopeptide B and Bradykinin in the transfer TWIG as a function of ND3 pressure. (b–c) Mass spectra of the singly-charged monomer (b) and the homodimer (c) of Leucine enkephalin at different ND3 pressures with a constant T-wave velocity of 300 m/s and a wave height of 6V. The dashed line in (a) corresponds to a deuterium uptake of 6 Da (as calculated from the average masses of the labeled and unlabeled species) and is also indicated in (b–c) for reference. Notably, the Leucine enkephalin dimer undergoes significantly less gas-phase labeling (decreased D uptake) than the monomer.