Figure 5.
(A) Co-exposure to a non-toxic concentration of NMDA (5 μM) and the polyamine spermidine (100 μM) produced significant toxicity in the CA1 region of hippocampal slice cultures. This toxicity was significantly reduced in cultures additionally exposed the NR2B-polyamine site specific antagonist ifenprodil (100 μM); **P < 0.05 vs. NMDA; #P < 0.05 vs. NMDA and spermidine. (B) Representative images of PI fluorescence following co-exposure to NMDA and spermidine or NMDA, spermidine and ifenprodil.