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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Biochem Pharmacol. 2010 Feb 15;79(4):565–574. doi: 10.1016/j.bcp.2009.09.020

Figure 7.

Figure 7

A) The effect of Nutlin-3 on the cell cycle when administered alone, or in combination with doxorubicin or 5FU. AJ02-NM0 cells were treated with Nutlin-3, 5FU or doxorubicin for 20 hours and the effect on the cell cycle distribution was analyzed by flow cytometry. The top panels show the impact of these agents relative to vehicle (DMSO)-treated control cells. The bottom panels show the cell cycle distribution of AJ02-NM0 cells treated with Nutlin-3 in combination with doxorubicin or 5FU. The asterisk indicates the sub-diploid cell population appearing in the cells treated with the doxorubicin plus Nutlin-3 combination. B) Increased caspase-3 activity indicates increased apoptosis following combination treatment of AJ02-NM0 cells with doxorubicin and Nutlin-3. AJ02-NM0 cells were treated with DMSO, doxorubicin, Nutlin-3, or a combination of doxorubicin and Nutlin-3 for 24 hours. Protein extracts were prepared and analyzed for caspase-3 activity using the Z-DEVD-AMC substrate.