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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Cell Signal. 2010 Mar;22(3):386–394. doi: 10.1016/j.cellsig.2009.10.008

Fig. 4. USP11 negatively regulates TNFα-mediated IL-6 gene expression.

Fig. 4

(A) Knockdown of USP11 expression enhances the TNFα-induced NF-κB-dependent IL-6 gene expression. The sh-Control, sh-USP11 cell lines were either untreated or treated with TNFα (1 ng/ml) for the time points indicated. Total RNAs from these cells were harvested. IL-6 transcript levels in the sh-Control and sh-USP11 cell lines were measured using qRT-PCR normalized to GAPDH. The data is presented as the average of three separate experiments with standard deviation. (B) Knockdown of USP11 expression enhances the TNFα-induced IL-6 production. The sh-Control, sh-USP11 cell lines were either untreated or treated with TNFα (5 ng/ml) for the time points indicated. The supernatants from these cell cultures were collected and subjected to the human IL-6 ELISA analysis according to the manufacturer’s instructions. (* p<0.01 versus respective control)