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. Author manuscript; available in PMC: 2010 Dec 22.
Published in final edited form as: Biochemistry. 2009 Dec 22;48(50):11982–11993. doi: 10.1021/bi901532z

Figure 4.

Figure 4

Tryptophan fluorescence spectra of hu1B7 (□), L-W91A (◆), H-W33A (x), PTx-S1-220 (Δ), and the hu1B7/PTx-S1-220 complex (◇) in PBS. A) hu1B7, L-W91A, and H-W33A comparison at 4.5 μM at room temperature. Lines indicate spectral peaks at 352 nm, 352 nm, and 355 nm, respectively. B) hu1B7, PTx-S1-220, and the hu1B7/PTx-S1-220 complex at a 1:1 molar ratio comparison at 4.5 μM at room temperature. Lines indicate spectral peaks at 357 nm, 362 nm, and 353 nm, respectively.