. Author manuscript; available in PMC: 2010 Dec 22.

Published in final edited form as: Biochemistry. 2009 Dec 22;48(50):11982–11993. doi: 10.1021/bi901532z

Table 1.

Binding between PTx, its subunits, and naturally occurring variants to 1B7

PTx	Mutations wt Tohama I (PTx-SIB)	ELISA^* %EC_{50 PTx}	m1B7 mAb ~ 1000 RU k_d × 10^–3 (sec⁻¹)
PTx	-	100	0.4 +/− 0.6
B-oligomer	-	8	2.7 +/− 0.6
PTx-S1 235	-	20^**	1.9 +/− 0.3
PTx-S1 220	-	90 +/− 10^***	1.4 +/− 0.1
PTx-S1A	M194I	50 +/− 40	0.71 +/− 0.04
PTx-S1D	D34E, I198V	90 +/− 50	0.95 +/− 0.05
PTx-S1E	D34E, S162P, I198M	80 +/− 30	1.8 +/− 0.5
PTx-S1 9K/129G	R9K, E129G	60 +/− 30	1.6 +/− 0.7

All ELISAs were run using hu1B7 as the primary antibody.

^**

S1 235 maintained in soluble form in 0.03% CHAPS, 0.1 mM Na₂EDTA, and 10 mM Tris

^***

Due to low expression, only the purest samples were used for these numbers